Bioinformatic Approaches for the Genetic and Phenotypic Characterization of a Saccharomyces Cerevisiae Wine Yeast Collection

phenotypic similarities within a group of 103 S cerevisiae strains that belong to the above mentioned strain Computational analysis Phenotypic tests Phenotyping included the evaluation of 19 traits used in yeast taxonomy [8] or for wine yeast strain selection. Yeast cells were withdrawn from frozen aliquots (glycerol, 30%, v/v, at -80oC) and were pre-inoculated in 10 ml of the culture medium to be used (see table) and incubated (24h, 30oC, 200rpm). Cell density was determined (OD640) and adjusted to 1.0. From this suspension, 15 μl were inoculated in 4 or 8 replicate wells of a 96-well microplate containing 135uL culture medium, so that the final cellular density was 0.1 OD. Growth conditions were used as indicated in the table . Carbon Sources Glucose